Student Learning Strategies
This process should be performed first, perhaps
even before starting the course. Each company may have its own manual
and procedures regarding sample collection. In order to correctly
draw a sample, which represents the entire fluid, certain steps should
be strictly followed, such as taking the sample midstream and not
from the top or bottom of the container.
After this activity, students will be able
- Draw samples into prepared bottles.
- Use sterile techniques to keep other components
or particles from contaminating the sample.
- Use prepared bottles for drawing the samples.
- Rinse your hands and the parts for sampling
with isopropyl alcohol.
- Place a sampling bucket under the outlet.
- Loosen (but not remove) the cap on the
- Open the outlet and let one half-gallon
into the bucket.
- Remove the cap and film.
- Place the bottle in the stream and collect
about 100 ml of the fluid.
- Remove the bottle from the stream and replace
the cap with its film.
- Close the outlet.
It is very important to dispose of the fluid
in the bucket properly.
- What should be used to clean hands and
the sampling parts?
- Why do you need to take the sample midstream
after a half-gallon is let into the bucket?
- Why do you close the outlet after the sample
bottle is removed?
Instructor Learning Strategies
Activity level of difficulty: Beginner
Suggested teaching strategies: Students
need a copy of these procedures before starting the class to draw
their samples before the wet lab day.
Time commitment: Overall, 10 to 15
- prepared bottles for drawing samples
- isopropyl alcohol
- alcohol dispenser (with filter)
- sampling bucket
- disposal container
Troubleshooting Tips: The use of sampling bottles
without a film in the cap will make it easier to reduce the chance
Answers to the Assessment Tools
- isopropyl alcohol.
- to ensure that we have a good representative
- so that the shavings from the equipment
and the outlet will not get into the sample.